Journal:

Article Title: Induction of T Helper Type 1 Responses by a Polysaccharide Deacetylase from Cryptococcus neoformans

doi: 10.1128/IAI.71.9.5412-5417.2003

Figure Lengend Snippet: rd25-induced IFN-γ and IL-2 production in cultures of splenocytes from rd25-immunized or C. neoformans-infected mice. Spleens were collected at 7, 14, and 28 days after immunization or infection (see the legend to Fig. ​Fig.1),1), and splenocytes (5 × 106/ml) were stimulated with CneF (10 μg/ml), rd25 (10 μg/ml), or PBS. Cytokine levels were assayed at 24 h (IL-2) or 48 h (IFN-γ) after the addition of the stimuli. Data are expressed as means ± SEM for three experiments, each performed in triplicate. Asterisk, P < 0.05, relative to results with cells from PBS-CFA-injected mice by one-way analysis of variance and the Student-Keuls-Newman test.

Article Snippet: To assess the role of IL-2, groups of d25-immunized and nonimmunized mice were treated with polyclonal goat anti-mouse IL-2 IgG (R&D Systems) or normal goat IgG and challenged with C. neoformans (7.5 × 10 3 CFU).

Techniques: Infection, Injection

Journal:

Article Title: Induction of T Helper Type 1 Responses by a Polysaccharide Deacetylase from Cryptococcus neoformans

doi: 10.1128/IAI.71.9.5412-5417.2003

Figure Lengend Snippet: rd25-induced IFN-γ and IL-2 production in cultures of CD4+ or CD8+ cells from C. neoformans-infected mice. Spleens were collected at 14 days after infection, and CD4+ or CD8+ T cells (5 × 106/ml), separated by means of a panning procedure (16), were cultured in the presence of splenic adherent cells (5 × 105/ml) and CneF (10 μg/ml), d25 (10 μg/ml), or PBS. Cytokine levels were assayed as indicated in the legend to Fig. ​Fig.2.2. Data are expressed as means ± SEM for three experiments, each performed in triplicate. Asterisk, P < 0.05, relative to results with cells from PBS-CFA-injected mice by one-way analysis of variance and the Student-Keuls-Newman test.

Article Snippet: To assess the role of IL-2, groups of d25-immunized and nonimmunized mice were treated with polyclonal goat anti-mouse IL-2 IgG (R&D Systems) or normal goat IgG and challenged with C. neoformans (7.5 × 10 3 CFU).

Techniques: Infection, Cell Culture, Injection

Journal:

Article Title: Induction of T Helper Type 1 Responses by a Polysaccharide Deacetylase from Cryptococcus neoformans

doi: 10.1128/IAI.71.9.5412-5417.2003

Figure Lengend Snippet: Role of IL-12 and TNF-α in the development of cytokine responses to d25. Spleens were collected at 7 and 14 days after immunization with rd25 (100 μg per mouse in CFA), and cultures of splenocytes (5 × 106/ml) were stimulated with rd25 (10 μg/ml). Cytokine levels were assayed at 24 h (IL-2) or 48 h (IFN-γ) after the addition of the stimulus. Experimental groups included immunized mice that were injected i.p. with 0.2 mg of goat polyclonal anti-mouse TNF-α IgG, goat anti-IL-12 IgG, or normal goat IgG at 1 h prior to infection. Data are expressed as means ± SEM for three experiments, each performed in triplicate. Asterisk, significantly (P < 0.05) different from normal IgG control group by one-way analysis of variance and the Student-Keuls-Newman test.

Article Snippet: To assess the role of IL-2, groups of d25-immunized and nonimmunized mice were treated with polyclonal goat anti-mouse IL-2 IgG (R&D Systems) or normal goat IgG and challenged with C. neoformans (7.5 × 10 3 CFU).

Techniques: Injection, Infection

Journal:

Article Title: Induction of T Helper Type 1 Responses by a Polysaccharide Deacetylase from Cryptococcus neoformans

doi: 10.1128/IAI.71.9.5412-5417.2003

Figure Lengend Snippet: Role of IL-2 and IFN-γ in the protective effects of d25 immunization. Panels A and B show the effects of, respectively, IL-2 blockade and IFN-γ deficiency on C. neoformans-induced lethality in d25-immunized and unimmunized mice. (A) Eight-week-old female BALB/c mice were immunized s.c. with rd25 (100 μg per mouse in CFA) or injected with PBS-CFA. Mice belonging to each of these two subgroups were injected i.p. at the time of immunization and 15 days later with 0.5 mg of goat polyclonal anti-mouse IL-2 IgG or normal goat IgG. All mice were challenged i.v. with 7.5 × 103 viable C. neoformans cells (strain H99) at 1 week after immunization, and lethality was observed daily for 30 days. (B) Eight-week-old female IFN-γ−/− mice on a C57BL/6 background and control WT C57BL/6 mice were immunized with d25 in CFA or injected with PBS-CFA as described above. After 1 week, mice were challenged i.v. with 3 × 104 viable C. neoformans cells (strain H99), and lethality was observed daily for 30 days. Survival data were analyzed with Kaplan-Meier survival plots followed by the log rank test (JMP Software; SAS Institute, Cary, N.C.) on an Apple Macintosh computer.

Article Snippet: To assess the role of IL-2, groups of d25-immunized and nonimmunized mice were treated with polyclonal goat anti-mouse IL-2 IgG (R&D Systems) or normal goat IgG and challenged with C. neoformans (7.5 × 10 3 CFU).

Techniques: Injection, Software

Journal: Scientific Reports

Article Title: Sexual dimorphic impacts of systemic vincristine on lower urinary tract function

doi: 10.1038/s41598-022-08585-3

Figure Lengend Snippet: Antibodies.

Article Snippet: IL-2 , St John's Laboratory (London, UK), STJ93688 , 1:1,000 (WB) , Rabbit , Fig. A .

Techniques: Biomarker Discovery